Advances in Microbial Physiology, Volume 19 by A.H. Rose (Editor), J. Gareth Morris (Editor)

By A.H. Rose (Editor), J. Gareth Morris (Editor)

This quantity in a research-level sequence covers diversified points of microbial body structure and biochemistry together with inositol metabolisms in yeasts, bacterial adhesion, natural acids, the bacterial flagellum and the mechanical behaviour of bacterial mobile partitions. it really is meant to be of use to microbiologists, biochemists and biotechnologists. different comparable works during this sequence are volumes 29, 30 and 31.

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1968) until after these suspensionswere spread on to the surface of agar-containing medium. It is not possible to say what was happening to wall formation when the inhibitors were present. , 1975b) of the agar medium on which the reverting protoplasts of B. lichenijomis had been incubated showed that autolytic degradation products of peptidoglycan had been secreted during the process. These products were eliminated in the early stages of reversion, and greatly reduced in quantity in the later stages, by including a cell-wall preparation in the reversion medium.

The poles ofB. subtilis, however, account for only about 15% of the peripheral wall instead of all of it as they do in streptococci. In one of these, the immunofluorescence method, as used by Cole and Hahn (1962), was applied to an autolytic deficient-phosphoglucomutase-negative strain of B. r (Hughes and Stokes, 1971); conserved zones of new wall were demonstrated in the centre of the cell or 25% along the length of the cells from the poles. Several aspects of this experiment differentiated it from other apparently similar ones with bacilli.

1969). When cells were disrupted, the autolytic activity in the soluble fraction was 6-10 times less than that in the soluble fraction from the parent. Isolated native walls of the mutant strain correspondingly had about 10 times more activity. Despite this somewhat confusing picture, deliberate isolation of mutants of Staph. , 1976). Thus, again in this mutant strain, there is a close direct relationship between the function of autolytic enzymes and separation of staphylococcal cells. The work of Koyama et al.

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